Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vitamin E changes the membrane fluidity of human platelets

M Steiner

    Biochimica Et Biophysica Acta
    |January 8, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Decline of caries prevalence in Swiss military recruits between 1970 and 1996].

    Schweizer Monatsschrift fur Zahnmedizin = Revue mensuelle suisse d'odonto-stomatologie = Rivista mensile svizzera di odontologia e stomatologia·2001
    Same author

    The selective serotonin reuptake inhibitor sertraline: its profile and use in psychiatric disorders.

    CNS drug reviews·2001
    Same author

    The efficacy of fluoxetine in improving physical symptoms associated with premenstrual dysphoric disorder.

    BJOG : an international journal of obstetrics and gynaecology·2001
    Same author

    Serum-to-urinary prostate-specific antigen ratio in patients with benign prostatic hyperplasia and prostate cancer.

    Anticancer research·2001
    Same author

    Aged garlic extract, a modulator of cardiovascular risk factors: a dose-finding study on the effects of AGE on platelet functions.

    The Journal of nutrition·2001
    Same author

    Diagnosis and treatment of premenstrual dysphoric disorder: an update.

    International clinical psychopharmacology·2001
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Alpha-tocopherol (vitamin E) alters human platelet membrane fluidity. This vitamin enrichment affects membrane protein interactions and flow activation energy, influencing cellular function.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Membrane Biophysics

    Background:

    • Alpha-tocopherol, a key antioxidant, is known to interact with cell membranes.
    • Understanding vitamin E's effects on platelet membrane properties is crucial for cellular health.

    Purpose of the Study:

    • To investigate the in vitro effects of alpha-tocopherol on human platelet membrane microviscosity and fluidity.
    • To elucidate the relationship between alpha-tocopherol levels and platelet membrane biophysical properties.

    Main Methods:

    • Utilized fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene-labeled human platelets and their membranes.
    • Measured changes in fluorescence anisotropy across a range of temperatures.
    • Analyzed liposomes prepared from platelet membrane lipids with and without alpha-tocopherol.

    Related Experiment Videos

    Main Results:

    • Alpha-tocopherol enrichment decreased fluorescence anisotropy below 24-27°C and increased it above this temperature.
    • Temperature-dependent anisotropy curves in enriched platelets showed a steeper slope, indicating increased flow activation energy.
    • Flow activation energy correlated linearly with platelet alpha-tocopherol levels.
    • Liposomes with alpha-tocopherol exhibited increased anisotropy, suggesting a role for membrane proteins.

    Conclusions:

    • Alpha-tocopherol significantly modulates the apparent microviscosity of human platelet membranes.
    • The observed changes in membrane fluidity and flow activation energy may be linked to interactions with membrane proteins.
    • This modulation by alpha-tocopherol could contribute to its overall physiological effects on human cells.