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Zinc uptake by isolated rat hepatocytes

N H Stacey, C D Klaassen

Biochimica Et Biophysica Acta

|February 6, 1981

Summary

This study reveals zinc uptake in rat hepatocytes occurs in two phases. The initial rapid phase appears carrier-mediated, while the second, slower phase does not involve active transport mechanisms.

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Area of Science:

Biochemistry
Cell Biology
Toxicology

Background:

Zinc is an essential trace element vital for numerous cellular functions.
Understanding zinc uptake mechanisms is crucial for cellular homeostasis and toxicity studies.

Purpose of the Study:

To investigate the early uptake kinetics of zinc in isolated rat hepatocytes.
To differentiate between carrier-mediated and active transport components in zinc uptake.

Main Methods:

Isolated rat hepatocytes were incubated with radio-labeled zinc (65Zn) at varying concentrations (1-500 microM).
Uptake was measured over early time points (25 s to 60 min).
The effects of cadmium, potassium cyanide (KCN), and carbonyl cyanide m-chlorophenylhydrazone (CCCP) on zinc uptake were assessed.

Main Results:

A biphasic zinc uptake pattern was observed: a rapid initial phase and a slower second phase.
The first phase showed nonlinear kinetics, suggesting carrier involvement, and was inhibited by cadmium.
The second phase exhibited linear kinetics, and zinc uptake was not affected by KCN or CCCP, indicating a lack of active transport.

Conclusions:

Hepatocyte zinc uptake involves a biphasic process with a carrier-mediated component in the initial rapid phase.
The slower, later phase of zinc uptake does not appear to be actively transported.
Cadmium specifically interferes with the carrier-mediated uptake of zinc.

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