[Factors causing oligomerization of wall-eyed pollock rhodopsin before and after solubilization by sodium dodecyl...

Using SDS-electrophoresis, it was shown that the addition of sodium ions to the solubilization medium causes oligomerization of wall-eyed pollock rhodopsin. Oligomerization is also caused by solubilization of the wall-eyed pollock rod outer segments by sodium cholate (deoxycholate), by changes in SDS concentration as well as by an increase of protein content in the solubilization mixture, freezing of solubilizate and treatment of the rod outer segments by sodium borohydrate upon illumination. Possible causes of wall-eyed pollock rhodopsin aggregation are discussed.