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Hybridization studies within the genus Schizosaccharomyces Lindner

Canadian Journal of Microbiology

|February 1, 1981

Summary

This study explored hybridization in the yeast genus Schizosaccharomyces using auxotrophic mutants. Researchers successfully recovered intrastrain and interstrain recombinants but found no successful crosses between different species.

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Area of Science:

* Microbiology
* Yeast genetics
* Molecular biology

Background:

* The genus Schizosaccharomyces comprises several yeast species with potential biotechnological applications.
* Understanding their reproductive compatibility is crucial for genetic manipulation and breeding.
* Auxotrophic mutants are valuable tools for selection-based hybridization studies.

Purpose of the Study:

* To investigate the potential for genetic recombination within and between species of the genus Schizosaccharomyces.
* To determine the feasibility of using prototrophic selection for identifying successful hybrid yeast strains.
* To assess the interspecific compatibility of six representative Schizosaccharomyces species.

Main Methods:

* Utilized the prototrophic selection technique with auxotrophic mutants from six Schizosaccharomyces species.
* Performed intrastrain crosses (within the same strain) and interstrain crosses (between different strains).
* Conducted extensive interspecific crosses (between different species) to evaluate reproductive barriers.

Main Results:

* Successfully recovered intrastrain recombinants in five out of six tested parental pairs.
* Identified interstrain recombinants from crosses involving Schizosaccharomyces japonicus and Schizosaccharomyces pombe.
* Observed no recombinant recovery in 62 interspecific crosses across all tested species combinations.

Conclusions:

* Genetic recombination is feasible within strains and between certain closely related strains of Schizosaccharomyces.
* Significant reproductive barriers exist between different species within the genus Schizosaccharomyces.
* Interspecific hybridization is not achievable using current methods in this genus, limiting potential breeding strategies.

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