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Serological diagnosis in children infected prenatally with lymphocytic choriomeningitis virus

M M Sheinbergas, V J Lewis, W L Thacker

    Infection and Immunity
    |February 1, 1981
    PubMed

    Insights

    Antibodies to Lymphocytic choriomeningitis virus were found in children years after suspected prenatal infection. However, a neutralization test did not detect these antibodies in all cases.

    Area of Science:

    • Virology
    • Immunology
    • Pediatrics

    Background:

    • Prenatal exposure to Lymphocytic choriomeningitis virus (LCMV) can occur.
    • The long-term immune response to prenatal LCMV infection in children is not well understood.

    Purpose of the Study:

    • To investigate the presence and detectability of LCMV antibodies in children with probable prenatal LCMV infection.
    • To compare immunofluorescence and neutralization tests for detecting LCMV antibodies in this cohort.

    Main Methods:

    • Sera from six children, collected 2 to 8 years postnatally, were tested for LCMV antibodies.
    • Immunofluorescence assay was used as the primary detection method.
    • A neutralization test was employed as a secondary or confirmatory method.

    Main Results:

    • LCMV antibodies were detected by immunofluorescence in all six children.
    • Antibodies were not detected by the neutralization test in two of the six children.
    • This suggests potential differences in sensitivity or specificity between the two assays for long-term LCMV antibody detection.

    Conclusions:

    • Immunofluorescence can detect LCMV antibodies in children years after probable prenatal infection.
    • Neutralization tests may not detect antibodies in all individuals with past LCMV exposure.
    • Further research is needed to clarify the long-term antibody profiles and diagnostic utility of different assays for prenatal LCMV exposure.

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