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Plasmids in Yersinia pestis

D M Ferber, R R Brubaker

    Infection and Immunity
    |February 1, 1981
    PubMed
    Summary

    Yersinia pestis strains producing pesticin or dependent on Ca2+ contain specific plasmids. Most Yersinia pestis isolates also possess a cryptic 65-megadalton plasmid, indicating common genetic elements.

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    Area of Science:

    • Microbiology
    • Genetics
    • Bacteriology

    Background:

    • Yersinia pestis is a Gram-negative bacterium responsible for plague.
    • Plasmids are extrachromosomal DNA elements that can confer important traits to bacteria.
    • Understanding the plasmid content of Yersinia pestis is crucial for studying its virulence and evolution.

    Purpose of the Study:

    • To characterize the plasmid profiles of different Yersinia pestis strains.
    • To identify plasmids associated with pesticin production and Ca2+ dependence.
    • To investigate the prevalence of cryptic plasmids in Yersinia pestis isolates.

    Main Methods:

    • Plasmid DNA extraction from Yersinia pestis isolates.
    • Agarose gel electrophoresis to determine plasmid sizes.
    • Comparison of plasmid profiles among different strains.

    Main Results:

    • Pesticinogenic Yersinia pestis strains harbored a ~6 megadalton plasmid.
    • Ca2+-dependent Yersinia pestis strains harbored a ~45 megadalton plasmid.
    • A cryptic 65-megadalton plasmid was found in most examined Yersinia pestis isolates.

    Conclusions:

    • Specific plasmids are associated with key virulence factors in Yersinia pestis.
    • The 65-megadalton plasmid is a common feature across various Yersinia pestis strains.
    • Plasmid analysis provides insights into the genetic diversity and pathogenicity of Yersinia pestis.

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