High-performance liquid chromatography of cardiac glycosides

Highly efficient, short columns have been prepared for the separation of mixtures of cardiac glycosides at relatively small pressure drops. The selectivities of silica adsorbents with different chemically modified surface layers for cardiac glycosides were determined. The influence of the structure of cardiac glycoside molecules on their retention characteristics in liquid chromatography was investigated. The relative number of hydrophobic and hydrophilic groups in the glycoside molecules as well as the configurations are very important for the separation.