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Updated: Aug 24, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 10, 2013
A method for determining the three-dimensional form of active flagella, using two-colour darkground illumination
Abstract:
Using darkground illumination, and placing colour filters over half the field, one can make a single, highly resolved, colour micrograph of a filamentous object (such as a mammalian sperm tail) from which it is possible to get a complete and unambiguous three-dimensional reconstruction.
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