Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Resolution in light microscope autoradiography using a carbon-14 labelled line source

C Vanroelen, F Harrisson, L Andries

    Journal of Microscopy
    |April 1, 1981
    PubMed
    Summary

    Thicker emulsion or section layers in light microscope autoradiographs reduce resolution, as measured by half-distance (HD). Autoradiograph distributions correlated well with electron microscope data, indicating reliable methodology for studying radioactive material distribution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    An exploratory, open-label, randomized, multicenter study to investigate the pharmacodynamics of a glycoengineered antibody (imgatuzumab) and cetuximab in patients with operable head and neck squamous cell carcinoma.

    Annals of oncology : official journal of the European Society for Medical Oncology·2017
    Same author

    Demonstration of the interaction between glycosaminoglycans and fibronectin in the basement membrane of the living chicken embryo.

    Wilhelm Roux's archives of developmental biology·2017
    Same author

    Precarious employment: understanding an emerging social determinant of health.

    Annual review of public health·2014
    Same author

    The cholecystokinin CCK2 receptor antagonist, JNJ-26070109, inhibits gastric acid secretion and prevents omeprazole-induced acid rebound in the rat.

    British journal of pharmacology·2012
    Same author

    Differential exposure and differential vulnerability as counteracting forces linking the psychosocial work environment to socioeconomic health differences.

    Journal of epidemiology and community health·2009
    Same author

    The influence of small field sizes, penumbra, spot size and measurement depth on perturbation factors for microionization chambers.

    Physics in medicine and biology·2009

    Area of Science:

    • Radiological imaging
    • Microscopy techniques
    • Nuclear medicine

    Background:

    • Autoradiography is crucial for visualizing the distribution of radioactive isotopes in biological samples.
    • Optimizing resolution in autoradiography is essential for accurate quantitative analysis.
    • Light microscopy and electron microscopy offer different resolutions for autoradiographic studies.

    Purpose of the Study:

    • To investigate the impact of emulsion and section thickness on resolution in light microscope autoradiographs.
    • To quantify autoradiographic resolution using the half-distance (HD) value.
    • To compare light microscope autoradiograph data with electron microscope autoradiograph data.

    Main Methods:

    • Preparation of light microscope autoradiographs using a Carbon-14 (14C) line source.

    Related Experiment Videos

  • Measurement of grain density distributions around the line source.
  • Quantification of resolution using the half-distance (HD) value.
  • Normalization of data and comparison between light and electron microscopy results.

    • Main Results:

    • Increased emulsion thickness and section thickness led to a higher HD value, indicating reduced resolution.
    • The shape of grain density distribution curves was similar across different thicknesses after normalization.
    • A good correlation was observed between normalized light microscope autoradiograph data and electron microscope autoradiograph data, except near the source.

    Conclusions:

    • Emulsion and section thickness are critical factors affecting resolution in light microscope autoradiography.
    • The half-distance (HD) value effectively quantifies resolution in this technique.
    • Light microscope autoradiography, when optimized, provides comparable distribution data to electron microscope autoradiography for quantitative analysis.