Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The cell substratum modulates skeletal muscle differentiation

H F Elson, J S Ingwall

    Journal of Supramolecular Structure
    |January 1, 1980
    PubMed
    Summary

    Hyaluronate (HA) inhibits skeletal muscle cell differentiation during chick embryogenesis. Degradation of HA in the extracellular matrix is necessary for proper myogenesis, suggesting a regulatory role for HA.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Energetics of the failing heart: new insights using genetic modification in the mouse.

    Archives des maladies du coeur et des vaisseaux·2006
    Same author

    Cardiac-specific expression of heme oxygenase-1 protects against ischemia and reperfusion injury in transgenic mice.

    Circulation research·2001
    Same author

    Endogenous nitric oxide enhances coupling between O2 consumption and ATP synthesis in guinea pig hearts.

    American journal of physiology. Heart and circulatory physiology·2001
    Same author

    Metabolic support as an adjunct to inotropic support in the hypoperfused heart.

    Journal of molecular and cellular cardiology·2001
    Same author

    Type 2 iodothyronin deiodinase transgene expression in the mouse heart causes cardiac-specific thyrotoxicosis.

    Endocrinology·2001
    Same author

    Phenotypical features of long Q-T syndrome in transgenic mice expressing human Na-K-ATPase alpha(3)-isoform in hearts.

    American journal of physiology. Heart and circulatory physiology·2000

    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Embryonic development involves significant changes in the extracellular matrix (ECM).
    • Components of the ECM may regulate cell differentiation processes.
    • Skeletal muscle differentiation is a key developmental event.

    Purpose of the Study:

    • To investigate the role of extracellular matrix components, specifically collagen and glycosaminoglycans, in regulating skeletal muscle cell differentiation.
    • To determine the effect of hyaluronate (HA) on myogenesis in a cell-culture system.

    Main Methods:

    • Utilized a cell-culture system derived from embryonic chick skeletal muscle tissue.
    • Studied the effects of collagen and glycosaminoglycans (including HA) on muscle cell attachment, spreading, and differentiation.
    • Measured inhibition of myogenesis and the muscle-specific M subunit of creatine kinase.
    • Conducted control experiments with other glycosaminoglycans and assessed general metabolic inhibition.

    Main Results:

    • Collagen was essential for muscle cell attachment and spreading.
    • Hyaluronate (HA) significantly inhibited myogenesis, particularly the M subunit of creatine kinase, without affecting cell attachment or spreading.
    • Inhibition by HA occurred only when added within the first 24 hours of culture, mirroring in vivo ECM degradation.
    • Other glycosaminoglycans did not cause this specific inhibition.

    Conclusions:

    • Hyaluronate (HA) plays a regulatory role in modulating skeletal muscle differentiation.
    • Degradation of inhibitory ECM components like HA is a prerequisite for successful myogenesis.
    • These findings highlight the dynamic nature of the ECM during embryogenesis and its influence on cell fate.

    Related Experiment Videos