[Effect of surface-active substances on hydroxylase activity of Tieghemella orchidis in a free and immobilized state]

The effect of nonionogenic surface-active substances (SAS) on hydroxylase activity of Tieghemella orchidis in free and polyacrylamide gel (PAAG) immobilized states was studied. It was shown that addition of SAS (1%) to the cultivation medium simultaneously with the inoculation stimulated the filamentous growth of the mycelium. It was also demonstrated that certain SAS (span-80/sorbitanemonooleate/and polyethylene glycol-600-monolaurate) affected beneficially the culture transformation activity. The hydroxylase activity also increased after mycelium preincubation with tweens (1%) and transformation in the presence of span-20 (sorbitane monolaurate) and span-80 (0.3%). The use of the latter substances during gel preincubation with immobilized cells led to the stabilization of transformation activity, increase in the hydroxylation rate, and a change in the yield of hydroxylated products to produce hydrocortisone.