[Complex isolation of intra- and extracellular RNases from Aspergillus clavatus]

A scheme for isolating guanyl-specific and -nonspecific intracellular and extracellular RNases from the fungus Aspergillus clavatus cultivated in a fermenter has been developed. The scheme involves chromatography on DEAE and CM-celluloses, QAE Sephadex A-50 gel filtration on Sephadex G-75 and ultragel A-44, and crystallization. Using the scheme, four electrophoretically homogeneous enzymes have been purified, two of them being crystalline guanyl-specific RNases and two nonspecific RNases.