A rapid screening method for the detection of monospecific antibodies against hemoglobins

A simple procedure for the detection of monospecific antibody against C57BL/6 mouse hemoglobin that would not cross-react with DBA/2 mouse hemoglobin is described. The horse antiserum against C57BL/6 mouse hemoglobin is absorbed with DBA/2 mouse hemoglobin. The absorbed serum is then allowed to react with small amounts of DBA/2 hemoglobin-Sepharose and C57BL/6 hemoglobin-Sepharose in separate tubes, followed by reaction with fluorescein isothiocyanate-conjugated goat antihorse IgG. A strong fluorescence in the C57BL/6 immunoabsorbent and little or no fluorescence in the DBA/2 immunoabsorbent show the presence of monospecific antibody against C57BL/6 hemoglobin. The method has general applicability as antibodies against other hemoglobins and proteins can be detected.