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Related Experiment Videos

Allophenic mice in cleft-palate investigations

K M Sulik, R L Atnip

    Journal of Embryology and Experimental Morphology
    |October 1, 1978
    PubMed
    Summary

    This study investigates cleft palate (CP) in T1Wh mice, finding cortisone acetate induces CP in all offspring. T1Wh mice exhibit a higher CP susceptibility than C57BL/6J mice.

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    Area of Science:

    • Developmental Biology
    • Genetics
    • Teratology

    Background:

    • Cleft palate (CP) is a common congenital anomaly.
    • The T1Wh mouse strain, characterized by a Robertsonian translocation, has not been previously studied for CP susceptibility.
    • Cortisone acetate is a known teratogen that can induce CP in mice.

    Purpose of the Study:

    • To investigate the susceptibility of T1Wh mice to cortisone-induced cleft palate.
    • To compare CP incidence and developmental timing between T1Wh and C57BL/6J mouse strains.
    • To explore the cellular basis of CP in allophenic T1Wh x C57BL/6J mouse models.

    Main Methods:

    • T1Wh and C57BL/6J mouse embryos were aggregated to create allophenic blastocysts.
    • Allophenic blastocysts were transferred to pseudopregnant recipients.
    • Pregnant recipients were treated with cortisone acetate during critical gestation periods (days 11-14).
    • Fetuses were examined on day 18 of gestation for CP and chromosomal analysis.

    Main Results:

    • All T1Wh x T1Wh allophenic fetuses exhibited CP.
    • Cleft palate occurred in 20% of C57BL/6J x C57BL/6J allophenic fetuses.
    • CP was observed in 26.2% of T1Wh x C57BL/6J allophenic fetuses.
    • A positive correlation was found between CP presence and the percentage of susceptible cells in the palate (P < 0.02).
    • C57BL/6J cells appeared to have a growth advantage in the palates of T1Wh x C57BL/6J allophenic mice.

    Conclusions:

    • T1Wh mice are highly susceptible to cortisone-induced cleft palate.
    • Cellular composition and potential cell-cell interactions influence CP development.
    • The T1Wh strain offers a valuable model for studying the genetic and cellular mechanisms of cleft palate.

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