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[The identification of nonfermentative gram-negative bacteria. Experiences with 676 apyocyaninogenic strains
Abstract:
During a period of 16 months 1757 strains of nonfermentative gram-negative rods have been isolated from clinical material. Of the, 1205 (69%) were P. aeruginosa, 124 (10%) of which failed to produce pyocyanin. The apyocyaninogenic strains as well as the remaining 552 isolates were differentiated by steps according to a diagnostic scheme developed by us. For identification of species two or three steps were needed. By this procedure, 530 of the 552 strains could be assigned to nineteen species within the genera Pseudomonas, Achromobacter, Alcaligenes, Flavobacterium, Agrobacterium and Acinetobacter. 17 strains could not be identified below the genus level, one strain belonged to CDC-group VE-2 and four strains were not identifiable. 72% of the 552 strains belonged to only four species: Pseudomonas putida, P. maltophilia, Acinetobacter lwoffii and A. anitratus.
Insights
This study identified 1757 nonfermentative gram-negative rods from clinical samples. A new diagnostic scheme efficiently identified most strains, with Pseudomonas and Acinetobacter species being most common.
Area of Science:
- Clinical Microbiology
- Bacteriology
Context:
- Nonfermentative gram-negative rods are frequently isolated from clinical specimens.
- Accurate identification of these bacteria is crucial for patient care and infection control.
Purpose:
- To develop and evaluate a diagnostic scheme for the identification of nonfermentative gram-negative rods.
- To determine the prevalence of different species within this group of bacteria.
Summary:
- A total of 1757 strains were isolated over 16 months, with Pseudomonas aeruginosa comprising 69%.
- A novel diagnostic scheme required 2-3 steps for species identification, successfully classifying 530 of 552 non-aeruginosa strains into 19 species.
- Pseudomonas putida, P. maltophilia, Acinetobacter lwoffii, and A. anitratus represented 72% of the identified strains.
Impact:
- Provides an efficient method for identifying clinically relevant nonfermentative gram-negative rods.
- Highlights the most common species encountered, aiding in targeted diagnostic and therapeutic strategies.
- Contributes to a better understanding of the microbial landscape in clinical settings.