Injury and repair of the lung: response to intravenous Freund's adjuvant

Tissue from the lungs of rabbits was examined at intervals up to 24 weeks after the animals had received a single intravenous injection of Freund's complete adjuvant. Though this is not a conventional method for damaging the peripheral lung, it had the advantage of producing multiple lesions in which most tissue components were altered for a prolonged period. White blood cells were present within the tissue and air spaces of these damaged areas. They persisted for 6 weeks in large numbers and gradually decreased over the next 12 weeks. There was replacement of type A by type B alveolar lining cells. Basement membranes were displaced and lost. Elastic and collagen fibres were distorted and destroyed. Blood vessels were occluded. Epithelioid cell and foreign body granulomas developed. Interalveolar septa disappeared, and air spaces were compressed. Despite all these changes the lungs regained near normal structure by 24 weeks after the initial injury. These results do not support the importance that has been placed on damage to various structural components of the lung as an explanation for chronic pulmonary disease. They do give some insight into the capacity of peripheral lung tissue for regenerationa following a single injury that induces a prolonged inflammatory response.