Phospholipid metabolism in Mycobacterium smegmatis ATCC 607 grown at 37 degrees and 27 C degrees C

The rate of synthesis and degradation of phospholipids in Mycobacterium smegmatis ATCC 607, grown at 27 degrees C and 37 degrees C was studied incorporation of 32P into phospholipids and chase of radioactivity of the pulse-labelled phospholipids. A relatively low rate of synthesis and degradation of phospholipids in cells growth at 27 degrees C was observed as compared to those grown at 37 degrees C. Phosphatidylethanolamine (PE) had the maximum turnover at 37 degrees C. However, at 27 degrees C, cardiolipin (CL) showed a turnover rate higher than PE. Phosphatidylinositol mannosides (PIMs) were metabolically more active at 37 degrees C than at 27 degrees C. The differences in metabolic activity of the phospholipids at the two temperatures have been discussed.