Related Experiment Video
Updated: Aug 17, 2026
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
Published on: December 12, 2011
[Problems relating to low temperature preservation of ram semen. 1. Effects of various cryoprotective agents on
Archiv Fur Experimentelle Veterinarmedizin
|January 1, 1981
Abstract:
Studies into the action of several cryoprotective agents in sodium-citrate-yolk diluent on ram semen provided preliminary information regarding both concentration applicable in deep-freeze preservation and toxicity of the agents involved. Included in the tests were glycerin, dimethylsulphoxide, ethylene glycol, propylene glycol, polyethylene glycol, polyvinylpyrrolidome, and dimethylacetamide. The effect of adding cryoprotective-containing diluent at 5 degrees C was more favourable, in its impact on ram sperma, than that observed, when diluent was added at 30 degrees C.
Related Concept Videos
01:41Spermatogenesis
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
01:28Cryo-electron Microscopy
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...