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Related Experiment Videos

The high mobility group proteins and transcribed nucleosomes

C G Mathew, G H Goodwin, C A Wright

    Cell Biology International Reports
    |January 1, 1981
    PubMed
    Summary

    Monomer nucleosomes released from nuclei contain specific high mobility group (HMG) proteins and are enriched in transcribed DNA sequences. Further analysis revealed heterogeneity and potential protein rearrangement within these nucleosomes.

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    Area of Science:

    • Molecular Biology
    • Chromatin Biology
    • Nucleosome Structure

    Background:

    • Monomer nucleosomes are released from nuclei following micrococcal nuclease digestion.
    • These released nucleosomes are known to be enriched in transcribed DNA sequences.
    • Previous studies indicated a depletion of histone H1 and enrichment of high mobility group (HMG) proteins in these nucleosomes.

    Purpose of the Study:

    • To characterize the protein composition of monomer nucleosomes released from nuclei.
    • To investigate the heterogeneity of these nucleosomes using polyacrylamide gel electrophoresis.
    • To determine if HMG-nucleosomes are specifically enriched in transcribed DNA sequences.

    Main Methods:

    • Micrococcal nuclease digestion of nuclei to release monomer nucleosomes.

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  • Analysis of nucleosome protein composition, focusing on H1 and HMG proteins (HMG14, HMG17).
  • Separation of nucleosomes by polyacrylamide gel electrophoresis (PAGE).

    • Main Results:

    • Released monomer nucleosomes were depleted in H1 and enriched in HMG14, HMG17, and another HMG-like protein.
    • PAGE analysis revealed heterogeneity among the released nucleosomes.
    • Nucleosomes containing HMG14 and HMG17 were identified.
    • However, HMG-nucleosomes from chick erythrocytes did not show enrichment in globin sequences.

    Conclusions:

    • Monomer nucleosomes released by nuclease digestion are distinct in protein composition, containing specific HMG proteins.
    • Nucleosome heterogeneity exists, with distinct populations binding HMG proteins.
    • The lack of globin sequence enrichment in HMG-nucleosomes suggests potential protein rearrangement or selective association.