Glycoprotein synthesis in colonic epithelial cells from rats

Viable colonic epithelial cells from rats were isolated by a non-enzymatic procedure using EDTA. The isolated cells were fractionated by sedimentation through at 15% to 35% discontinuous Ficoll gradient to yield cells differing in proliferative capacity from three different density regions of the gradient. Glycoprotein synthesis of these fractionated cells was examined in terms of their ability to absorb and incorporate labeled glucosamine and fucose into trichloracetic acid precipitable material. Glycoprotein synthesis was highest among cells with intermediate densities, banding in the middle of the gradient (Fraction II). Cytomorphological examination showed that these cells were predominantly goblet type.