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Pinocytic uptake of divinyl ether-maleic anhydride (pyran copolymer) and its failure to stimulate pinocytosis

M K Pratten, R Duncan, H C Cable

    Chemico-Biological Interactions
    |June 1, 1981
    PubMed

    Insights

    DIVEMA, a pyran copolymer, and its derivatives did not enhance pinocytosis in rat cells. Instead, these compounds were rapidly accumulated by macrophages, suggesting an adsorptive pinocytic mechanism.

    Area of Science:

    • Pharmacology
    • Cell Biology
    • Biomaterials Science

    Background:

    • Pinocytosis is a crucial cellular process for uptake of extracellular material.
    • DIVEMA (pyran copolymer) and its derivatives are potential biomaterials.
    • Previous research suggested potential enhancement of pinocytosis by DIVEMA.

    Purpose of the Study:

    • To investigate the effect of DIVEMA and its derivatives on cellular pinocytic uptake.
    • To determine the uptake mechanism of DIVEMA by macrophages.

    Main Methods:

    • In vitro study using rat visceral yolk sac and peritoneal macrophages.
    • Utilized 125I-labeled polyvinylpyrrolidone (PVP) and colloidal 198Au as markers for pinocytosis.
    • Employed [14C]DIVEMA and 125I-labelled DIVEMA to track compound accumulation.

    Main Results:

    • DIVEMA and its derivatives did not enhance the pinocytic uptake of PVP or colloidal gold.
    • Inhibition of pinocytosis was observed in some experimental conditions.
    • [14C]DIVEMA and 125I-labelled DIVEMA showed rapid accumulation in rat peritoneal macrophages.

    Conclusions:

    • DIVEMA and its derivatives do not stimulate pinocytosis.
    • Macrophage accumulation of DIVEMA occurs via an adsorptive pinocytic mechanism.
    • Findings contradict earlier expectations regarding DIVEMA's effect on pinocytosis.

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