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Related Experiment Videos

Competition between platelets and lung for 5-hydroxytryptamine uptake

H Steinberg, D K Das

    Experimental Lung Research
    |June 1, 1980
    PubMed
    Summary
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    Pulmonary endothelial cells and platelets uptake serotonin (5-HT) in circulation. Lung endothelium removes most 5-HT, but platelets also contribute, especially when aggregated or under low oxygen.

    Area of Science:

    • Pulmonary circulation
    • Vascular biology
    • Pharmacology

    Background:

    • Pulmonary endothelial cells clear circulating serotonin (5-HT) via uptake and metabolism.
    • Platelets are a major source of 5-HT and a potential site for its uptake.

    Purpose of the Study:

    • To investigate platelet uptake of 5-HT within the pulmonary circulation.
    • To compare 5-HT uptake by pulmonary endothelium versus platelets.

    Main Methods:

    • Isolated ventilated rat lungs were perfused with various plasma preparations (buffer, platelet-poor, platelet-rich, thrombin-treated).
    • Radioactive 5-HT ((14C) 5-HT) was infused, and lung and platelet uptake were measured.
    • Platelet function (counts, aggregation) was assessed post-circulation.

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    Main Results:

    • The lung removed approximately 65-68% of infused 5-HT in buffer or platelet-poor plasma.
    • In platelet-rich plasma, lung removal decreased to 51%, with platelets accounting for 30% of uptake.
    • Aggregated or low-oxygen-exposed platelets showed reduced 5-HT uptake (24% and 15%, respectively).

    Conclusions:

    • Pulmonary endothelium is the primary site for 5-HT removal in the pulmonary circulation.
    • Platelets contribute to 5-HT uptake, with their capacity influenced by activation state and oxygen tension.
    • Circulating 5-HT is cleared by either endothelial metabolism or platelet reuptake.