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Vascular changes in rabbit skin induced by proinflammatory phorbol and 12-deoxyphorbol esters
Inflammation
|March 1, 1981
Summary
Certain phorbol and 12-deoxyphorbol esters cause vasoconstriction in rabbit skin. These compounds did not significantly increase plasma exudation alone but did when combined with PGE1.
Area of Science:
- Pharmacology
- Dermatology
- Vascular Biology
Background:
- Phorbol esters are known for their diverse biological activities.
- Understanding their effects on microvasculature is crucial for pharmacological applications.
Purpose of the Study:
- To investigate the effects of specific phorbol and 12-deoxyphorbol esters on rabbit skin blood flow and plasma exudation.
- To determine the dose-dependent vasoconstrictive and exudative responses.
Main Methods:
- Blood flow measured using 133Xe washout technique.
- Plasma exudation quantified by 131I-labeled human serum albumin leakage.
- In vitro studies using superfused rabbit aorta for vasoconstriction assessment.
Main Results:
- 12-Deoxyphorbol esters (phenylacetate, angelate) and their C-20 acetates induced significant vasoconstriction at 100 ng/site.
- Tetradecanoyl phorbol acetate showed weaker vasoconstriction.
- Phorbol and 9,13,14-orthophenylacetyl-resiniferonol had no significant effect.
- Plasma exudation was not increased by esters alone but was elevated (up to 40 microliter/site) when combined with PGE1.
Conclusions:
- Specific tigliane esters possess potent vasoconstrictive properties in rabbit skin and aorta.
- These esters do not significantly induce plasma exudation independently.
- Synergistic effects on plasma exudation observed when combined with prostaglandin E1 (PGE1).