Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hexagonal glycoprotein arrays from Sindbis virus membranes

C H von Bonsdorff, S C Harrison

    Journal of Virology
    |November 1, 1978
    PubMed
    Summary

    The structure of Sindbis virus glycoproteins remains consistent, regardless of the internal nucleocapsid. This finding highlights the independent nature of glycoprotein interactions in viral assembly.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    An experimental comparison of two methods for warming intravenous crystalloid solutions.

    Anaesthesia·2019
    Same author

    A role forAmanita muscaria L. in the circulation of cadmium and vanadium in a non-polluted woodland.

    Environmental geochemistry and health·2013
    Same author

    Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant.

    Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2013
    Same author

    Molecular structures and interactions in the yeast kinetochore.

    Cold Spring Harbor symposia on quantitative biology·2011
    Same author

    Climate change and the integrity of science.

    Science (New York, N.Y.)·2010
    Same author

    Detection of human norovirus from frozen raspberries in a cluster of gastroenteritis outbreaks.

    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2009

    Area of Science:

    • Virology
    • Structural Biology
    • Biochemistry

    Background:

    • Sindbis virus is an enveloped virus with a nucleocapsid core and a surrounding membrane studded with glycoproteins.
    • Understanding the structural organization of viral glycoproteins is crucial for comprehending viral assembly and entry mechanisms.

    Purpose of the Study:

    • To investigate the influence of the Sindbis virus nucleocapsid on the structural arrangement of viral glycoproteins.
    • To determine if glycoprotein-glycoprotein interactions within the viral membrane are dependent on the underlying nucleocapsid.

    Main Methods:

    • Freeze-etch electron microscopy was employed to visualize the surface and internal structures of Sindbis virus particles.
    • Glycoprotein arrays were isolated from Sindbis virus membranes using nonionic detergent treatment for further analysis.

    Main Results:

    • Electron microscopy revealed the detailed local geometry of glycoprotein-glycoprotein interactions on the viral surface.
    • These interactions were observed to be structurally consistent in both intact Sindbis virus and in isolated glycoprotein arrays, independent of the nucleocapsid.

    Conclusions:

    • The local geometry of Sindbis virus glycoprotein interactions is independent of the presence of the nucleocapsid.
    • This suggests that the self-assembly or structural organization of viral glycoproteins can occur without direct interaction with the internal viral core.

    Related Experiment Videos