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Extraction of light filth from tea: collaborative study

F F Lim, J Barnett, A Bright

Journal - Association of Official Analytical Chemists

|March 1, 1981

Summary

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A new, faster method for analyzing light filth in tea significantly improves insect and rodent fragment recovery rates. This AOAC official first action method reduces sample analysis time from 27 to 9 minutes.

Area of Science:

Food Science
Analytical Chemistry
Entomology

Background:

The established AOAC method for detecting insect and rodent filth in tea is inefficient due to time-consuming filter paper analysis.
Heavy plant residue on filter papers prolongs the reading time, necessitating a more efficient analytical approach.

Purpose of the Study:

To develop and validate a novel, improved method for the quantitative analysis of light filth in tea.
To enhance recovery rates of insect fragments and rodent hairs while reducing analysis time.

Main Methods:

A new tea analysis protocol involving sample preparation, wet sieving, 40% isopropanol dilution, and mineral oil-heptane flotation.
Utilized a Wildman trap flask for efficient trapping of light filth during analysis.
Conducted an interlaboratory collaborative study to assess the method's performance and reliability.

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Main Results:

The proposed method achieved a 99.2% recovery rate for insect fragments, compared to 93.0% for the AOAC method.
Rodent hair recovery was significantly higher with the proposed method (92.2%) versus the AOAC method (47.6%).
Average analysis time per subsample decreased from 27 minutes (AOAC) to 9 minutes (proposed method).

Conclusions:

The newly developed method offers superior efficiency and accuracy for light filth analysis in tea.
The proposed method has been officially adopted by AOAC as a first action method.
This advancement streamlines quality control processes in the tea industry.