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Human parathyroid hormone: antibody characterization

R Hehrmann, J P Nordmeyer, H Mohr

    Journal of Immunoassay
    |January 1, 1980
    PubMed
    Summary
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    New parathyroid hormone (PTH) antibodies were developed in sheep, showing cross-reactivity with human, bovine, and porcine PTH. These antibodies are valuable for both clinical radioimmunoassays and experimental research.

    Area of Science:

    • Endocrinology
    • Immunology
    • Biochemistry

    Background:

    • Parathyroid hormone (PTH) plays a crucial role in calcium and phosphate homeostasis.
    • Development of specific antibodies is essential for accurate PTH measurement and research.

    Purpose of the Study:

    • To generate and characterize sheep polyclonal antibodies against parathyroid hormone (PTH).
    • To assess the cross-reactivity and affinity of these antibodies for different PTH species and fragments.

    Main Methods:

    • Immunization of two sheep (S 469 and S 478) with porcine and bovine parathyroid extracts.
    • Characterization of antisera using various PTH preparations and fragments.
    • Affinity determination using radioimmunoassay techniques.

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    Main Results:

    • Both antisera exhibited cross-reactivity with human, bovine, and porcine PTH; one also bound rat PTH.
    • Antibody binding was primarily directed towards the mid-region (44-68 fragment) of the PTH molecule.
    • Sheep S 478 showed high affinity for intact hormone, while S 469 demonstrated higher affinity for the 44-68 fragment.

    Conclusions:

    • Developed sheep antibodies are effective tools for detecting PTH across multiple species.
    • The antibodies demonstrate high affinity and regional specificity, suitable for radioimmunoassay and experimental applications.
    • These antibodies have been distributed for broader laboratory use in PTH research.