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Related Experiment Videos

Wing buds with three ZPAs

L E Iten, D J Murphy, L C Javois

    The Journal of Experimental Zoology
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Grafting extra ZPA tissue into embryonic chick wings did not increase morphogen concentration as hypothesized. Instead, greater positional disparity between grafted and host tissues led to more extra limb structures, challenging previous models.

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    Area of Science:

    • Developmental biology
    • Embryology
    • Limb development

    Background:

    • The zone of polarizing activity (ZPA) is crucial for embryonic limb development.
    • A diffusible morphogen from the ZPA is hypothesized to control limb patterning.
    • Cells' fates are thought to be respecified by morphogen concentration.

    Purpose of the Study:

    • To test the hypothesis that increasing ZPA tissue increases morphogen concentration and respecifies cell fate.
    • To investigate the relationship between ZPA grafting and the formation of extra limb structures.

    Main Methods:

    • Grafting experiments involving the zone of polarizing activity (ZPA) in embryonic chick wing buds.
    • Analysis of the resulting limb structures to determine the effect of ZPA grafts.

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    Main Results:

    • Attempts to increase morphogen concentration by grafting additional ZPAs failed to induce the predicted respecification.
    • A positive correlation was observed between the positional disparity at graft sites and the formation of supernumerary limb structures.

    Conclusions:

    • The simple model of morphogen concentration directly correlating with respecification may be incomplete.
    • Positional information and tissue interactions play a significant role in generating extra limb structures during development.