Application of Ca2+-selective electrode for determination of Ca2+ efflux from smooth muscles into Ca2+-free...

The Ca2+ efflux from smooth muscle into a Ca2+-free physiological solution was continuously monitored by using a Radiometer Ca2+-selective electrode and examined for its availability for determination of Ca2+ effluxed from the smooth muscle. The results show that the Ca2+ electrode used here is sufficiently sensitive to investigate Ca2+ efflux from the smooth muscle into the Ca2+-free solution. Further, this method is suitable for the continuous recording of free ionized Ca2+ effluxed from the smooth muscle into the Ca2+-free physiological solution.