Related Experiment Video
Updated: Oct 11, 2026

Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine
Published on: October 7, 2014
The effect of phencyclidine on noradrenaline uptake by bovine chromaffin granules
Abstract:
The effects of the psychotomimetic drug, phencyclidine, on the reserpine sensitive uptake of (-)noradrenaline, on the reserpine resistant uptake of tryptamine, and on catecholamine release were studied in vitro using bovine chromaffin granules. Phencyclidine inhibited the uptake of (-)noradrenaline and tryptamine in a concentration-dependent manner. It caused 50% inhibition of (-)noradrenaline uptake at 2 X 10(-4)M and of tryptamine uptake at 7 X 10(-4)M. Release of catecholamines was not affected by phencyclidine at 0 degree C and pH 6--8 in concentrations up to 8 X 10(-3)M, whereas at 37 degrees C the drug (4 X 10(-3)M) caused a release that was increased when the pH was raised from 6 to 8. Since the effects of phencyclidine on chromaffin granule uptake and release are observed at high concentrations of the drug only, there is no evidence that these effects are relevant to the in vivo effects of the drug.
More Related Videos
Related Concept Videos
Desensitization and Tachyphylaxis
Several...
Adrenergic Neurons: Neurotransmission
Synthesis: Catecholamine synthesis requires tyrosine, which is taken...
Adrenergic Receptors: β Subtype
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors have equal affinities for...
Adrenergic Agonists: Indirect-Acting Agents
One mechanism involves depleting stored catecholamines by displacing them from synaptic vesicles. These agents, known as "displacers," are transported into vesicles at the expense of noradrenaline. Examples include amphetamine and tyramine, which lack a catechol moiety, resulting in prolonged action, improved oral bioavailability, and...
Drugs Affecting Neurotransmitter Release or Uptake
Drugs Affecting Neurotransmitter Synthesis

