[Use of direct spectrophotometry for the quantitative analysis of corticosteroids on thin-layer chromatograms]

Direct densimetry at 252.5 microns of cortisol on chromatographic plates with a thin silica gel layer provides the best results in comparison with fluorimetry, that includes pretreatment of plates with tetrazole reagent. Accuracy, sensitivity and reproducibility of the method were examined with reference to quantitative determination of the blood serum corticosterone of rats. Owing to a high sensitivity the direct densimetry technique in reflected light at 252.5 microns proved suitable for quantitative evaluation of the steroid spectrum in extracts from the rat adrenal incubate sections and the human blood serum. 3 ml of the blood serum is required for analysis performance.