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Related Experiment Videos

Endotoxin-induced tumor necrosis factor

H F Oettgen, E A Carswell, R L Kassel

    Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progres Dans Les Recherches Sur Le Cancer
    |January 1, 1980
    PubMed
    Summary

    Researchers discovered tumor necrosis factor (TNF) in BCG-infected mice treated with endotoxin. This host-released factor, TNF, causes tumor necrosis and is toxic to cancer cells in vitro.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Oncology

    Background:

    • Endotoxin administration to BCG-infected mice induces a serum factor with tumor-necrotizing properties.
    • This factor, termed tumor necrosis factor (TNF), mimics endotoxin's effects but is host-derived.

    Purpose of the Study:

    • To characterize the nature and properties of the newly identified tumor necrosis factor (TNF).
    • To investigate the selective toxicity of TNF towards neoplastic cells.

    Main Methods:

    • Induction of TNF in mice, rats, and rabbits via BCG infection and endotoxin treatment.
    • In vitro assays to assess TNF's cytotoxicity against various cell lines, including neoplastic and normal cells.
    • Biochemical analysis to determine TNF's molecular weight and composition.

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    Main Results:

    • TNF was identified as a glycoprotein with a molecular weight under 70,000.
    • TNF demonstrated selective toxicity, causing necrosis of transplanted murine tumors and in vitro cytotoxicity against murine and human cancer cell lines.
    • TNF did not exhibit toxicity towards mouse embryo cultures.
    • TNF exhibited distinct in vitro immunomodulatory effects, some similar to and others different from endotoxin.

    Conclusions:

    • Tumor necrosis factor (TNF) is a host-derived mediator responsible for endotoxin-induced tumor necrosis.
    • TNF possesses selective cytotoxic activity against cancer cells, making it a potential therapeutic agent.
    • Further research into TNF's immunomodulatory functions is warranted.