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Updated: Oct 17, 2026

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
Published on: August 25, 2014
Myelination of central nervous system axons in tissue culture by transplanted oligodendrocytes
Abstract:
Unmyelinated mouse cerebellar cerebellar cultures in which oligodendrocyte differentiation had been suppressed by exposure to cytosine arabinoside developed axonal myelin after superimposition of kainic acid-treated cerebellar explants devoid of myelin-receptive axons. The latter explants contained differentiated oligodendrocytes. The operation of a diffusible myelin-stimulating factor was ruled out by the failure of myelination in cytosine arabinoside-exposed explants not in direct contact with oligodendrocyte-containing transplants.
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