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Related Experiment Videos

Quality control for deglycerolized red blood cells

H T Meryman, M Hornblower

    Transfusion
    |May 1, 1981
    PubMed
    Summary

    A simple test can detect inadequately deglycerolized red blood cells, ensuring patient safety. While routine use requires cost-benefit analysis, this quality control method is feasible for blood banks.

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    Storage parameters affecting red blood cell survival and function after transfusion.

    Transfusion medicine reviews·1999

    Area of Science:

    • Blood banking and transfusion medicine
    • Cellular cryopreservation

    Background:

    • Glycerol is used to protect red blood cells (RBCs) during freezing.
    • Inadequate removal of glycerol can lead to intravascular hemolysis upon transfusion.
    • Quality control is essential to ensure the safety of transfused RBCs.

    Purpose of the Study:

    • To evaluate the feasibility of a quality-control procedure for detecting inadequately deglycerolized RBCs.
    • To assess the effectiveness of a simple method for identifying residual glycerol in deglycerolized RBCs.

    Main Methods:

    • Resuspending deglycerolized RBCs in 0.7% NaCl.
    • Estimating the percentage of hemolysis using a color comparator.

    Main Results:

    • The proposed quality-control procedure is feasible for detecting inadequately deglycerolized RBCs.
    • The test is simple and effective when performed correctly.
    • The risk of inadequate deglycerolization is low with meticulous adherence to standard protocols.

    Conclusions:

    • A quality-control test for deglycerolized RBCs is feasible and effective.
    • The cost-effectiveness of routine implementation should be evaluated by individual blood banks.
    • Ensuring proper deglycerolization minimizes the risk of transfusion-related hemolysis.

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