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Related Experiment Videos

Sampling procedures in estrogen receptor determinations

J F Newsome, F P Avis, J E Hammond

    Annals of Surgery
    |May 1, 1981
    PubMed
    Summary

    Delayed freezing and proteolysis significantly decrease estrogen receptor (ER) levels in tumor samples. Prompt freezing of fresh, histologically confirmed tumor tissue is crucial for accurate ER measurements.

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    Area of Science:

    • Oncology
    • Biochemistry
    • Pathology

    Background:

    • Estrogen receptor (ER) levels are critical biomarkers in breast cancer treatment decisions.
    • Accurate ER quantification is essential for selecting appropriate endocrine therapies.

    Purpose of the Study:

    • To investigate the impact of proteolysis and delayed freezing on ER levels in tumor samples.
    • To identify critical factors affecting the reliability of ER measurements.

    Main Methods:

    • Comparison of ER values between biopsy specimens and matched postmastectomy tumor tissue.
    • Time-decay studies on postmastectomy samples exposed to room temperature.
    • Analysis of factors including tumor cell concentration and necrosis.

    Main Results:

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    • A significant decrease (15-100%) in ER content was observed in postmastectomy samples compared to initial biopsies.
    • Further reduction in ER levels occurred with increased time between devascularization and freezing.
    • ER levels showed an inverse relationship with the duration of sample exposure to room temperature.

    Conclusions:

    • Proteolysis and delayed freezing substantially degrade ER levels, compromising measurement accuracy.
    • Timely processing and prompt freezing of fresh, histologically confirmed tumor specimens are vital for reliable ER quantification.