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Related Experiment Videos

The fluctuation test

B A Bridges

    Archives of Toxicology
    |November 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    The bacterial fluctuation test detects chemical-induced mutations using a color change in liquid medium. This sensitive assay is comparable to the Ames test for mutagenicity screening.

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    Area of Science:

    • Microbiology
    • Toxicology
    • Genetics

    Background:

    • The Ames test is a standard bacterial assay for detecting chemical mutagens.
    • Assays for mutagenicity are crucial for assessing chemical safety and carcinogenicity.
    • Bacterial mutation detection assays inform risk assessment for human health.

    Purpose of the Study:

    • To evaluate the fluctuation test as an alternative bacterial mutagenicity assay.
    • To compare the fluctuation test's performance with the established Ames test.
    • To highlight the advantages and limitations of the fluctuation test for mutagen detection.

    Main Methods:

    • Utilizes a liquid medium-based bacterial assay scoring color change (yellow).
    • Employs bacterial strains like Ames Salmonella or Escherichia coli WP2 trp.

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  • Incorporates metabolic activation using S9 fraction or hepatocytes.

    • Main Results:

    • The fluctuation test demonstrates comparable ability to the Ames test in detecting mutagens and carcinogens.
    • It shows slightly greater sensitivity and potential for automation.
    • The test is particularly effective for aqueous samples with low mutagen levels.

    Conclusions:

    • The fluctuation test is a viable alternative to the Ames test for bacterial mutagenicity detection.
    • Its sensitivity and automation potential offer advantages for specific applications.
    • The assay is well-suited for screening aqueous samples for low-level mutagens.