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Different mechanisms of synthesis of complete and incomplete influenza virus transcripts in infected cells
Archives of Virology
|January 1, 1981
Abstract:
Two types of transcripts, incomplete (polyadenylated virus-specific mRNAs) and complete (presumably templates for progeny virus replication) are synthesized in influenza virus infected cells (3). The synthesis of incomplete transcripts requires as a primer de novo synthesized host cell mRNAs (4). We show here that the synthesis of complete transcripts does not require such a primer.
Insights
Influenza virus infection produces two RNA transcripts. Complete transcript synthesis, essential for viral replication, does not require a host cell mRNA primer, unlike incomplete transcripts.
Area of Science:
- Virology
- Molecular Biology
- RNA Synthesis
Background:
- Influenza virus infection involves the synthesis of two distinct viral RNA transcripts.
- Incomplete transcripts (polyadenylated virus-specific mRNAs) require host cell mRNA primers for synthesis.
- Complete transcripts are presumed templates for progeny virus replication.
Purpose of the Study:
- To investigate the primer requirement for the synthesis of complete influenza virus transcripts.
- To differentiate the mechanisms of complete and incomplete viral RNA synthesis.
Main Methods:
- Analysis of viral RNA synthesis in influenza virus-infected cells.
- Distinguishing between complete and incomplete viral RNA transcripts.
Main Results:
- Demonstrated that complete influenza virus transcripts are synthesized independently of host cell mRNA primers.
- Confirmed that incomplete transcripts necessitate de novo synthesized host cell mRNAs as primers.
Conclusions:
- The synthesis of complete influenza virus RNA transcripts follows a distinct pathway compared to incomplete transcripts.
- Complete transcripts are likely directly involved in viral genome replication due to their primer-independent synthesis.