[Determination of lactic acid in blood by enzymatic-spectrophotometry method in normal subjects]

The authors carried out duplicate dosages of lactic acid in 20 normal patients (11 females and 9 males) ages ranged from 17 to 48 years. The spectrophotometric method was employed, and the values obtained from 0.69 to 4.21 mMol/L with a mean value of 1.5095 +/- 0.975. Some of the difficulties of the method and how to avoid them are discussed.