Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Very low density lipoprotein binding to adipocytes

K S Desai, G Steiner, I Takeuchi

    Biochimica Et Biophysica Acta
    |December 5, 1980
    PubMed
    Summary

    Human very low density lipoprotein (VLDL) binds to adipocytes via a specific receptor. This receptor interacts with VLDL and other lipoproteins, differing from the LDL receptor.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Spectroscopic investigation of the middle ear mucosa].

    HNO·2020
    Same author

    Current approaches to avoid the culling of day-old male chicks in the layer industry, with special reference to spectroscopic methods.

    Poultry science·2018
    Same author

    Production of neutral molecular clusters by controlled neutralization of mobility standards.

    Aerosol science and technology : the journal of the American Association for Aerosol Research·2017
    Same author

    [Diffuse idiopathic skeletal hyperostosis in the dog (DISH): a review].

    Schweizer Archiv fur Tierheilkunde·2016
    Same author

    From autoantibody research to standardized diagnostic assays in the management of human diseases - report of the 12th Dresden Symposium on Autoantibodies.

    Lupus·2016
    Same author

    Pathological brain plasticity and cognition in the offspring of males subjected to postnatal traumatic stress.

    Molecular psychiatry·2014

    Area of Science:

    • Lipid metabolism
    • Cell biology
    • Adipocyte research

    Background:

    • Very low density lipoprotein (VLDL) is a key lipoprotein in lipid transport.
    • Adipocytes play a crucial role in energy storage and metabolism.

    Purpose of the Study:

    • To investigate the interaction between human very low density lipoprotein (VLDL) and adipocytes.
    • To characterize the binding properties and specificity of VLDL receptors on adipocytes.

    Main Methods:

    • Incubation of isolated adipocytes and adipocyte membranes with radiolabeled VLDL.
    • Competition assays using various lipoproteins (HDL, LDL) and apolipoproteins.
    • Scatchard plot analysis and dissociation studies.

    Main Results:

    • VLDL binding to adipocytes is saturable, reversible, and dependent on time and temperature.
    • Human adipocytes have a higher VLDL binding capacity than rat adipocytes.
    • VLDL binding sites interact with HDL and C apolipoproteins, but not LDL, apolipoprotein B, or apolipoprotein A.

    Conclusions:

    • Adipocytes possess specific binding sites for VLDL.
    • The VLDL receptor on adipocytes differs from the LDL receptor, showing affinity for C apolipoproteins rather than apolipoprotein B or E.

    Related Experiment Videos