Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phytohemagglutinin isolectin subunit composition

R L Felsted, R D Leavitt, C Chen

    Biochimica Et Biophysica Acta
    |March 27, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Tuberculous tenosynovitis and bursitis: imaging findings in 21 cases.

    Radiology·1996
    Same author

    Congenital coronary arteriovenous fistula: spontaneous rupture and cardiac tamponade.

    The Annals of thoracic surgery·1996
    Same author

    Recombinant mitotoxin basic fibroblast growth factor-saporin reduces venous anastomotic intimal hyperplasia in the arteriovenous graft.

    Circulation·1996
    Same author

    Measurement of urinary estrogen metabolites using a monoclonal enzyme-linked immunoassay kit: assay performance and feasibility for epidemiological studies.

    Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·1996
    Same author

    Differences in cholinergic responses from outer hair cells of rat and guinea pig.

    Hearing research·1996
    Same author

    Pharmacokinetics of retinoids in women after meal consumption or vitamin A supplementation.

    Journal of clinical pharmacology·1996
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Phytohemagglutinin isolectins from red kidney beans were analyzed using electrophoresis. Different methods revealed unique subunit compositions for each isolectin, confirming their structures.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Plant Science

    Background:

    • Phytohemagglutinin (PHA) is a lectin found in kidney beans (Phaseolus vulgaris).
    • PHA exists as multiple isolectins with varying subunit compositions.
    • Understanding these compositions is crucial for studying lectin function and interactions.

    Purpose of the Study:

    • To determine the precise subunit compositions of individual PHA isolectins.
    • To differentiate between homotetrameric and intermediate PHA isolectins.
    • To validate the structural heterogeneity of PHA.

    Main Methods:

    • Isoelectric focusing (IEF) on polyacrylamide gels.
    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using continuous and discontinuous buffer systems.

    Related Experiment Videos

  • Absorbance scanning for quantitative analysis of protein bands.

    • Main Results:

    • IEF demonstrated unique, non-overlapping band patterns for homotetrameric isolectins (E4 and L4).
    • Discontinuous SDS-PAGE resolved E4 and L4 into distinct protein bands with specific molecular weights.
    • Intermediate isolectins (E3L1, E2L2, E1L3) showed a combination of bands, confirming their mixed subunit composition.

    Conclusions:

    • The study successfully elucidated the subunit stoichiometry of PHA isolectins (E4, E3L1, E2L2, E1L3, L4).
    • IEF and discontinuous SDS-PAGE are effective methods for distinguishing PHA isolectin heterogeneity.
    • These findings provide a detailed molecular basis for PHA isolectin diversity.