Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ca2+-accumulation in experimental spinal cord trauma

R D Happel, K P Smith, N L Banik

    Brain Research
    |May 4, 1981
    PubMed
    Summary

    Spinal cord injury in rats significantly elevates calcium levels within 45 minutes, peaking at 8 hours post-trauma. This quantitative study confirms calcium deposits in axons following experimental spinal cord trauma.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Role of Calpain in Spinal Cord Injury: Effects of Calpain and Free Radical Inhibitors<sup>a</sup>.

    Annals of the New York Academy of Sciences·2017
    Same author

    Abnormal glycosylation of myelin-associated glycoprotein in quaking mouse brain.

    Neurochemistry international·2010
    Same author

    Acylation of myelin proteolipid protein in subcellular fractions of rat brainstem.

    Neurochemistry international·2010
    Same author

    Molecular and immunogenic features of myelin lipids: incitants or modulators of multiple sclerosis?

    Multiple sclerosis (Houndmills, Basingstoke, England)·2009
    Same author

    Maternal alcohol consumption increases sphingosine levels in the brains of progeny mice.

    Neurochemical research·2007
    Same author

    Relatively low levels of calpain expression in juvenile rat correlate with less neuronal apoptosis after spinal cord injury.

    Experimental neurology·2004

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Trauma Research

    Background:

    • Spinal cord trauma is a severe injury with significant neurological consequences.
    • Previous studies suggested calcium deposits within axons in the lesion area.

    Purpose of the Study:

    • To quantitatively measure the time course of calcium levels following experimental spinal cord trauma.
    • To correlate calcium level changes with known morphological findings.

    Main Methods:

    • Experimental spinal cord injury was induced in rats using a standardized weight-drop model.
    • Lumbar spinal cord sections (lesioned and control) were analyzed for calcium content.
    • Atomic absorption spectroscopy was employed for quantitative calcium measurements.

    Related Experiment Videos

    Main Results:

    • Total calcium levels in the lesioned spinal cord were significantly elevated compared to control values.
    • Elevated calcium levels were observed as early as 45 minutes post-trauma.
    • Maximal increase in calcium levels was recorded at 8 hours after the injury.

    Conclusions:

    • Quantitative data confirm a rapid and significant increase in calcium levels following spinal cord trauma.
    • The findings support the hypothesis of calcium accumulation within axons in the traumatized spinal cord.
    • This study provides crucial biochemical evidence for the role of calcium dysregulation in spinal cord injury pathophysiology.