Radiometric enzyme-inhibition technique for measuring acivicin in plasma

A sensitive radiometric enzyme-inhibition assay is described for the determination of acivicin in plasma; it is based on the potent inhibition of carbamyl phosphate synthetase II (CPS) by the drug. Plasma is heated at 95 degrees C for 5 minutes to quantitatively detach bound acivicin. After centrifugation, free drug is quantitated by exposing purified CPS from Escherichia coli to representative aliquots or subdilutions of the resultant supernatants in the presence of L-glutamine, L-aspartic acid, ATP-MgCl2, NaH[14C]O3, and purified L-aspartate transcarbamylase (ATC) from E. coli. Carbamyl phosphate is first synthesized from L-glutamine, ATP-MgCl2, and NaH[14C]O3 by the action of CPS. The unstable carbamyl phosphate thus generated is quickly and quantitatively converted to [14C]carbamyl-L-aspartic acid by the action of ATC utilizing [14C]carbamyl phosphate and L-aspartic acid as substrates. After a 15-minute incubation at 37 degrees c, unreacted NaH[14C]O3 is dissipated at acidic pH and the newly formed [14C]carbamyl-L-aspartic acid is quantitated by scintillation spectrometry. The percent inhibition of the formation of carbamyl-L-aspartic acid through the conjoint actions of CPS and ATC responds in a linear way to the logarithm of the concentration of acivicin between 20 and 200 microM. The unknown concentration of acivicin is determined indirectly by matching the percent inhibition produced by the unknown to the percent inhibition produced by a series of acivicin standards extending over the linear range. This assay is sensitive, adequately reproducible, and easy. It can be used to measure acivicin in the plasma of subjects treated with this new oncolytic agent.