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Related Experiment Videos

Automated particle-counting immunoassay for digoxin

D Collet-Cassart, C G Magnusson, C L Cambiaso

    Clinical Chemistry
    |July 1, 1981
    PubMed
    Summary

    A new automated immunoassay accurately measures serum digoxin levels using rheumatoid factor, offering a non-radioisotopic method for therapeutic drug monitoring. This particle-counting technique achieves high throughput and reliable results, correlating well with radioimmunoassay.

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    Area of Science:

    • Clinical Chemistry
    • Immunology
    • Analytical Chemistry

    Background:

    • Accurate serum digoxin monitoring is crucial for therapeutic drug management.
    • Existing methods like radioimmunoassay may have limitations.
    • Development of rapid, non-radioisotopic assays is desirable.

    Purpose of the Study:

    • To develop and validate a non-radioisotopic automated immunoassay for serum digoxin quantification.
    • To assess the assay's performance characteristics, including speed, accuracy, and precision.

    Main Methods:

    • Utilized a particle-counting immunoassay technique with rheumatoid factor.
    • Digoxin levels were determined by its inhibition of digoxin-coated latex particle agglutination.
    • Employed pepsin digestion to mitigate interference from serum proteins.

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    Main Results:

    • Achieved an assay throughput of 50 samples per hour.
    • Demonstrated strong correlation with radioimmunoassay (r = 0.94) for samples between 0 and 5.7 µg/L.
    • Reported high analytical recovery (102%) and good precision (CVs ranging from 4.4% to 12.5%).

    Conclusions:

    • The developed automated immunoassay is a viable, non-radioisotopic alternative for serum digoxin measurement.
    • The assay provides accurate and precise quantification within the therapeutic range.
    • This method offers efficient therapeutic drug monitoring with high throughput.