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Ionized calcium analyzer with a built-in pH correction

N Fogh-Andersen

    Clinical Chemistry
    |July 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new apparatus for measuring free calcium ion and pH concentrations simultaneously. The device provides accurate, pH-corrected calcium measurements, crucial for clinical diagnostics.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Medical Devices

    Background:

    • Accurate measurement of free calcium ion concentration is vital for diagnosing metabolic bone diseases and other calcium-related disorders.
    • Existing methods for measuring calcium ion concentration can be complex and time-consuming.
    • Simultaneous measurement of pH and calcium ion concentration is necessary due to their interdependent relationship.

    Purpose of the Study:

    • To develop and validate a novel semi-automated apparatus for the simultaneous measurement of free calcium ion and hydrogen ion (pH) concentrations.
    • To assess the accuracy and precision of the new apparatus for clinical applications.
    • To provide a pH-corrected value for free calcium ion concentration at a standard pH of 7.40.

    Main Methods:

    Related Experiment Videos

  • A semi-automated apparatus was designed for simultaneous measurement of free calcium ion and pH at 37°C.
  • The apparatus utilizes ion-selective electrodes for calcium and pH determination.
  • Sample volume required is minimal (110 µL).
  • Calculations include actual concentrations and a pH-corrected free calcium ion concentration (at pH 7.40).

    • Main Results:

    • The apparatus demonstrated high precision with a within-series analytical standard deviation (SD) of 12 µmol/L and a day-to-day SD of 21 µmol/L for pH-corrected free calcium ion.
    • Mean serum free calcium ion concentrations were measured in healthy individuals (1.232 mmol/L) and patients (1.192 mmol/L).
    • The built-in pH correction accurately reflected the mean dependency on pH observed in patient sera.

    Conclusions:

    • The new semi-automated apparatus provides accurate and precise simultaneous measurements of free calcium ion and pH.
    • The pH-corrected calcium ion concentration is a valuable output for clinical interpretation.
    • This apparatus offers a reliable tool for assessing calcium status in various clinical settings.