Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Barbiturates--structure versus RIA reactivity

R D Budd, F C Yang, K O Utley

    Clinical Toxicology
    |March 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Malignant embryonal rhabdomyosarcoma with heterologous differentiation in gastric stromal tumors: report of a case].

    Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
    Same author

    [Mid-term clinical outcomes of supraspinatus tendon reconstruction versus superior capsular reconstruction for the treatment of irreparable posterosuperior massive rotator cuff tears].

    Zhonghua yi xue za zhi·2025
    Same author

    [Primary immunodeficiency disease based on ITK mutation: report of a case].

    Zhonghua bing li xue za zhi = Chinese journal of pathology·2024
    Same author

    [Efficacy analysis of surgical combined with postoperative adjuvant therapy for T3 gallbladder carcinoma: a multicenter retrospective study].

    Zhonghua wai ke za zhi [Chinese journal of surgery]·2023
    Same author

    Analysis of patient attitudes and behavior regarding dermatologic care during the COVID-19 pandemic: a survey-based study at a single academic institution.

    Dermatology online journal·2023
    Same author

    [Clinical value of extended radical resection for pT3 stage gallbladder cancer].

    Zhonghua yi xue za zhi·2020
    Same journal

    Chronic central nervous system toxicity of the chickling pea (Lathyrus sativus).

    Clinical toxicology·1981
    Same journal

    Brain energy metabolism in mice exposed to oxygen at 1 atmosphere absolute.

    Clinical toxicology·1981
    Same journal

    Toxicity of some essential plant oils. Clinical and experimental study.

    Clinical toxicology·1981
    Same journal

    Carbon disulfide neuropathy in rats. A morphological and ultrastructural study of degeneration and regeneration.

    Clinical toxicology·1981
    Same journal

    Regional differences in brain-soluble acetylcholinesterase and its molecular forms after acute poisoning by isoflurophate in rats.

    Clinical toxicology·1981
    Same journal

    Industrial neuropathy due to n-hexane. Clinical and morphological findings in three cases.

    Clinical toxicology·1981
    See all related articles

    This study analyzed over 90 barbituric acid compounds using radioimmunoassay (RIA). The 5,5-dialkyl barbituric acid ring structure is essential for reactivity, with modifications reducing binding affinity.

    Area of Science:

    • Medicinal Chemistry
    • Pharmacology
    • Analytical Chemistry

    Background:

    • Barbituric acid derivatives are a significant class of compounds with diverse pharmacological activities.
    • Radioimmunoassay (RIA) is a sensitive method for quantifying substances, including small molecules.
    • Understanding structure-activity relationships is crucial for drug design and development.

    Purpose of the Study:

    • To investigate the structural requirements for binding to radioimmunoassay (RIA) reagents targeting barbituric acid derivatives.
    • To compare the reactivity of various barbituric acid analogs with specific RIA antibodies.
    • To identify key structural features influencing the affinity of these compounds in an RIA assay.

    Main Methods:

    • Analysis of over 90 compounds structurally related to barbituric acid.

    Related Experiment Videos

  • Utilized radioimmunoassay (RIA) with 125I-secobarbital reagents.
  • Compared binding affinities based on molecular structures.

    • Main Results:

    • The 5,5-dialkyl barbituric acid ring structure is essential for reactivity with the employed RIA reagents.
    • Any structural modification at any position of the barbituric acid ring reduced reactivity.
    • No analyzed compounds exhibited higher reactivity than secobarbital or RO-2-1126.
    • Alterations in the 5-allyl and/or 5-(1-methylbutyl) groups of secobarbital led to diminished reactivity.

    Conclusions:

    • The integrity of the 5,5-dialkyl barbituric acid core is critical for RIA detection using the Roche reagents.
    • Specific substituents at the 5-position significantly impact binding affinity.
    • This research provides insights into the molecular recognition of barbituric acid derivatives in immunoassays, relevant for drug discovery and analysis.