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Cyclic erythropoiesis in W/Wv mice following a single small dose of 89Sr
Abstract:
Young adult female W/Wv mice were given 0.5 microCi 89Sr/g intravenously, a dose which produces no anemia and only mild transient thrombocytopenia in normal mice. In the W/Wv animals platelet counts fell from 10(6) to 3 x 10(5)/mm3, and hematocrits from 39% to 25% in two weeks. In the following 2 weeks, platelet counts rose to 7 x 10(5), stabilizing at this level. Average hematocrit values were observed to oscillate from a nadir of 26% to a zenith of 42%, with a periodicity of about 16 days. In a repeat experiment we found the average hematocrit fluctuation from 28 to 40%, amplitude of reticulocyte fluctuation 6 to 31%, periodicity of cycle 16 days. Several animals have been observed through as many as six complete cycles. Further study of cyclical erythropoiesis in the W/Wv mouse following hematopoietic injury produced by 89Sr may shed light on the causes of cyclical hematopoiesis observed occasionally in man and other animals.
Insights
Strontium-89 (89Sr) induced hematopoietic injury in W/Wv mice resulted in cyclical changes in platelet and hematocrit levels. This study reveals a novel model for understanding cyclical hematopoiesis.
Area of Science:
- Hematology
- Radiobiology
- Mouse Models
Background:
- W/Wv mice possess a defect in hematopoietic stem cells.
- Strontium-89 (89Sr) is a radiostrontium isotope used to induce hematopoietic injury.
Purpose of the Study:
- To investigate the hematological response in W/Wv mice following 89Sr administration.
- To characterize the cyclical patterns of hematopoiesis in this model.
Main Methods:
- Young adult female W/Wv mice were intravenously injected with 0.5 microCi 89Sr/g.
- Platelet counts, hematocrits, and reticulocyte levels were monitored over time.
- Cyclical patterns and periodicity were analyzed.
Main Results:
- 89Sr induced significant thrombocytopenia and decreased hematocrit in W/Wv mice.
- Hematocrit levels exhibited oscillations with a periodicity of approximately 16 days.
- Reticulocyte counts also showed cyclical fluctuations, mirroring hematocrit changes.
Conclusions:
- W/Wv mice treated with 89Sr demonstrate a reproducible model of cyclical hematopoiesis.
- This model may provide insights into the mechanisms underlying cyclical hematopoiesis in humans and other animals.
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