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Related Concept Videos

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

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In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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Related Experiment Video

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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Scleromalacia perforans in ulcerative colitis

P J Tesar, J A Burgess, J A Goy

    Gastroenterology
    |July 1, 1981
    PubMed
    Summary

    Ulcerative colitis can cause serious eye problems like scleromalacia perforans. This case report details successful treatment of this rare complication in a patient with active ulcerative colitis.

    Area of Science:

    • Ophthalmology
    • Gastroenterology
    • Rheumatology

    Background:

    • Ulcerative colitis (UC) is a chronic inflammatory bowel disease.
    • Ocular complications are common in UC patients.
    • Scleromalacia perforans, a rare condition causing thinning and potential perforation of the sclera, is typically associated with rheumatoid arthritis.

    Observation:

    • A 36-year-old male with a history of long-standing ulcerative colitis presented with active disease.
    • The patient developed scleromalacia perforans during a period of active ulcerative colitis.

    Findings:

    • This case highlights ulcerative colitis as a potential systemic disease associated with scleromalacia perforans.
    • The patient's scleromalacia perforans was effectively managed.

    More Related Videos

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    09:54

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    Published on: August 10, 2012

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    Last Updated: Jan 8, 2026

    Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
    09:35

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    Published on: January 20, 2015

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    Primary Culture of Mouse Dopaminergic Neurons
    11:58

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    Published on: September 8, 2014

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    Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
    09:54

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    Implications:

    • This finding expands the known systemic associations of scleromalacia perforans beyond rheumatoid arthritis.
    • Early recognition and management of ocular complications in UC are crucial.
    • Treatment involved high-dose steroids and surgical intervention (total proctocolectomy).