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Related Experiment Videos

Ketone kinetics in man

J A Bradley, R Swaminathan, G L Hill

    Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
    |March 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

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    This study compared ketone body kinetics in normal volunteers. It found that calculating metabolic clearance rate using injected ketone kinetics provides a more accurate estimate of total ketone body production than previous methods.

    Area of Science:

    • Biochemistry
    • Human Physiology
    • Metabolic Research

    Background:

    • Ketone bodies, including beta-hydroxybutyrate (D-beta-OHB) and acetoacetate (AcAc), are crucial metabolic fuels.
    • Accurate measurement of ketone body production and clearance is essential for understanding metabolic states.
    • Previous methods for estimating metabolic clearance rate (MCR) of ketones may have limitations.

    Purpose of the Study:

    • To compare the kinetics of injected DL-beta-hydroxybutyrate and acetoacetate in normal volunteers.
    • To evaluate different methods for calculating the metabolic clearance rate (MCR) of total ketone bodies.
    • To determine the most accurate method for estimating total ketone body production rate.

    Main Methods:

    • Five healthy volunteers received intravenous injections of DL-beta-hydroxybutyrate and acetoacetate on separate occasions.

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  • Blood concentrations of D-beta-OHB and AcAc were measured at 5-minute intervals for 40 minutes post-injection.
  • Calculated kinetic parameters: rate constant of disappearance (K), volume of distribution (V), and metabolic clearance rate (MCR).

    • Main Results:

    • Kinetic parameters (K, V, MCR) were similar for both injected DL-beta-hydroxybutyrate and acetoacetate.
    • MCR calculated from injected ketone kinetics was approximately double that calculated using previous methods.
    • The discrepancy in MCR values was attributed to differences in the apparent volume of distribution.

    Conclusions:

    • The kinetics of injected beta-hydroxybutyrate and acetoacetate can be used to estimate total ketone kinetics.
    • Calculating MCR from injected ketone kinetics provides a more accurate assessment than methods relying on total ketone changes.
    • The product of MCR of the injected ketone and basal total ketone concentration is suggested as the best estimate for total ketone body production rate.