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Colorimetric determination of disulfiram in tablets: collaborative study
Journal - Association of Official Analytical Chemists
|May 1, 1981
Summary
A new colorimetric method using cuprous iodide and disulfiram was validated for drug quantification in tablets. This official method provides accurate and reliable disulfiram (DSF) determination in pharmaceutical formulations.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Accurate quantification of disulfiram (DSF) is crucial for pharmaceutical quality control.
- Existing analytical methods may require further validation for routine use.
Purpose of the Study:
- To collaboratively study and validate a new colorimetric method for disulfiram determination in tablet form.
- To establish an official, reliable analytical procedure for DSF quantification.
Main Methods:
- Disulfiram was extracted from tablet matrices using methylene chloride.
- The extracted disulfiram reacted with cuprous iodide, and absorbance was measured colorimetrically.
- Method validation included assays on synthetic preparations and commercial tablets.
Main Results:
- Average recoveries of 98.4% and 99.2% were achieved for synthetic disulfiram.
- Commercial tablets (250 and 500 mg declared) showed mean values of 246.6 ± 6.40 mg and 493.6 ± 12.0 mg.
- Results closely correlated with the official NF iodometric titration procedure.
Conclusions:
- The colorimetric method using cuprous iodide and disulfiram is accurate and reliable for tablet analysis.
- This validated method has been adopted as official first action.
- The study supports the method's utility in pharmaceutical quality control settings.