Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
  1. Home
  3. Research Domains
  1. Home
  3. Research Domains

Related Concept Videos

  • Agricultural, Veterinary And Food Sciences
  • Veterinary Sciences
  • Veterinary Medicine (excl. Urology)
  • Role Of Gallbladder Mucus Hypersecretion In The Evolution Of Cholesterol Gallstones
  • Agricultural, Veterinary And Food Sciences
  • Veterinary Sciences
  • Veterinary Medicine (excl. Urology)
  • Role Of Gallbladder Mucus Hypersecretion In The Evolution Of Cholesterol Gallstones

    • Related Experiment Videos

    Role of gallbladder mucus hypersecretion in the evolution of cholesterol gallstones

    S P Lee, J T LaMont, M C Carey

      The Journal of Clinical Investigation
      |June 1, 1981

      View abstract on PubMed

      Summary
      This summary is machine-generated.

      High cholesterol in bile stimulates gallbladder mucin secretion, a key factor in gallstone formation. This gallbladder mucus acts as a nucleating agent, promoting cholesterol crystal development in bile.

      Related Experiment Videos

      Area of Science:

      • Gastroenterology
      • Biochemistry
      • Pathophysiology

      Background:

      • Mucin glycoproteins play a potential role in gallstone development.
      • Understanding the relationship between biliary lipids, mucin secretion, and gallstone formation is crucial.

      Purpose of the Study:

      • To investigate the link between biliary lipids, gallbladder mucin secretion, and gallstone formation.
      • To determine if increased cholesterol in bile stimulates gallbladder mucin hypersecretion and contributes to gallstone nucleation.

      Main Methods:

      • Organ culture of prairie dog gallbladder explants.
      • Incorporation of radiolabeled glucosamine to quantify glycoprotein synthesis.
      • Gel filtration to analyze secreted glycoprotein size.
      • Analysis of bile composition and cholesterol saturation.

      Main Results:

      • Cholesterol feeding significantly increased gallbladder mucin glycoprotein synthesis and secretion.
      • Peak mucin secretion occurred at 5 days, with a fivefold increase.
      • Gallbladder mucin gels induced nucleation of cholesterol liquid crystals, leading to gallstone formation.
      • Organ-specific and diet-dependent nature of mucin hypersecretion was confirmed.

      Conclusions:

      • Increased cholesterol in bile stimulates gallbladder mucus hypersecretion.
      • Gallbladder mucus acts as a potent nucleating agent for biliary cholesterol.
      • This mechanism contributes significantly to cholesterol gallstone formation.