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Chemotherapy sensitivity testing in human tumours

H G Richmond, R W Billington

    Journal of Clinical Pathology
    |April 1, 1981
    PubMed
    Summary

    Researchers explored establishing in vitro growth for 245 malignant tumors. They found varying success with different methods, achieving epithelial cell growth in 63 cases and observing diverse sensitivities to cytotoxic agents.

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    Area of Science:

    • Oncology
    • Cell Biology
    • Histopathology

    Background:

    • Establishing reliable in vitro models for malignant tumors is crucial for cancer research and drug development.
    • Routine histopathology provides a source of diverse tumor samples for experimental investigation.

    Purpose of the Study:

    • To assess the feasibility of establishing in vitro cell growth from a consecutive series of malignant tumors.
    • To evaluate the sensitivity of established tumor cell cultures to cytotoxic agents.

    Main Methods:

    • Utilized three disaggregation procedures: mechanical separation, trypsin digestion, and collagenase digestion.
    • Cultured resulting cell fractions in monolayer to establish in vitro growth.
    • Tested the sensitivity of successfully cultured cells to various cytotoxic agents.

    Main Results:

    • Achieved abundant epithelial cell growth in monolayer culture from 63 out of 245 malignant tumors.
    • Demonstrated variable sensitivity to cytotoxic agents among different tumor types and even within individual patient lesions.
    • Observed no indiscriminate cytotoxic effect, highlighting tumor-specific responses.

    Conclusions:

    • In vitro growth of malignant tumors can be established using different disaggregation methods, with varying success rates.
    • In vitro sensitivity testing shows promise for predicting patient response to cytotoxic agents, though further development is needed.
    • Future development of systems correlating in vitro and in vivo sensitivity could enable routine in vitro testing for solid tumors.

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